Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: List of markers used for immunohistochemistry

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Concentration Assay, Plasmid Preparation

Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: Visualization of the treated area, the transition zone, and untreated area as direct comparison by SD-OCT and histology at post-operative day 4. Hematoxylin and eosin (HE) staining already reveals atrophy of the photoreceptor outer segments and the outer nuclear layer (ONL) limited to the scraping site. The inner nuclear layer (INL) is not affected. Immunohistochemistry staining of the rabbit retina shows proliferating cells detected via Ki67 staining (red) which colocalize in part with subretinal microglia/macrophages shown by isolectin B4 staining (yellow). A strong deposition of collagen IV (red) is found around the scraping site beneath the ONL. Pan cytokeratin staining (red) for RPE already appears at the scraping site. Cell nuclei are stained with DAPI (cyan). Scale bar for SD-OCT = 200 µm, for histological images = 100 µm

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Staining, Immunohistochemistry

Journal: American Journal of Physiology - Renal Physiology

Article Title: Urinary extracellular vesicle-associated MCP-1 and NGAL derived from specific nephron segments differ between calcium oxalate stone formers and controls

doi: 10.1152/ajprenal.00515.2018

Figure Lengend Snippet: Numbers of MCP-1-, NGAL-, and OPN-associated urinary extracellular vesicles from the different segmental epithelium of nephrons in nonstone formers (controls) and idiopathic calcium stone formers

Article Snippet: Phycoerythrin (PE)-conjugated mouse anti-human MCP-1 (catalog no. 502604, BioLegend, San Diego, CA), rabbit anti-human NGAL (catalog no. bs-0444R-PE, Bioss Antibodies, Woburn, MA), mouse anti-human OPN (catalog no. IC14331P, R&D Systems, Minneapolis, MN), FITC-conjugated recombinant annexin V (marker for vesicles carrying surface phosphatidylserine, catalog no. 556419, BD Pharminagen, San Jose, CA), rabbit anti-human aquaporin-1 (marker of the simple squamous epithelium of the descending limb of Henle, catalog no. orb15121, Biorbyt, Cambridge, UK), rabbit anti-human aquaporin-2 (marker of principal cells of the collecting duct, catalog no. orb13806, Biorbyt), rabbit polyclonal anti-human urate transporter (URAT)1 (marker of the simple cuboidal epithelium of the proximal convoluted tubule, catalog no. bs-10357R-FITC, Bioss Antibodies], mouse anti-human uromodulin (marker of the thick ascending loop of Henle, catalog no. LS-B3105, Lifespan Biosciences, Seattle, WA), rabbit polyclonal anti-human SLC12A3 (marker of the simple cuboidal epithelium of the distal convoluted tubule, catalog no. bs-7694R-FITC, Bioss Antibodies), and rabbit anti-human cytokeratin-19 (marker of the transitional epithelium of the papillary duct, catalog no. bs-2190R-FITC, Bioss Antibodies) antibodies were used in this study.

Techniques: Marker, Biomarker Assay